alpha-Endorphin Antibody Conjugated to Biotin

Description

alpha-Endorphin Antibody Conjugated to Biotin Research Use Only

alpha-Endorphin Antibody Conjugated to Biotin is a biotinylated polyclonal antibody specific for -endorphin, an endogenous opioid peptide derived from pro-opiomelanocortin (POMC). Supplied as a 50 g solution, this high-affinity biotin conjugate enables sensitive streptavidin-based detection of -endorphin in immunoassays, immunohistochemistry, and neuroendocrine research.

Catalog number:	B2015909
Lot number:	Batch dependent
Expiration Date:	Batch dependent
Amount:	50 g
Molecular Weight or Concentration:	N/A
Supplied as:	Solution
Applications:	ELISA, radioimmunoassay (RIA), immunohistochemistry (IHC), immunocytochemistry, and streptavidin-biotin detection of -endorphin
Storage:	-20 C
Keywords:	alpha-Endorphin, Endorphin, Endorphin a
Grade:	Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um.

Scientific Overview

-Endorphin is an endogenous opioid peptide generated from the precursor pro-opiomelanocortin (POMC). It exhibits opioid activity primarily through – and -opioid receptors and is involved in pain modulation, stress response, and neuroendocrine regulation. The biotinylated antibody allows high-sensitivity detection and localization of -endorphin in neural tissues, plasma, and other biological samples using streptavidin-based amplification systems.

This alpha-Endorphin Antibody Biotin Conjugate is suitable for:

• Immunohistochemical mapping of -endorphin in the central nervous system
• Quantification of -endorphin by ELISA or radioimmunoassay
• Studies of opioid peptide systems, stress axis, and pain pathways
• Investigation of endorphin immunoreactivity in physiological and pathological conditions
• Neuropeptide co-localization and neuroendocrine research

Usage & Handling Guidance

Store the antibody solution at -20 C. Avoid repeated freezethaw cycles by preparing single-use working aliquots. Thaw gently on ice and mix by gentle inversion before use. Dilute in buffer containing carrier protein (e.g., 1% BSA) immediately prior to assay.

• Recommended applications: ELISA, RIA, IHC, and streptavidin-biotin detection systems
• Working dilution: Optimize empirically for each tissue, fixation method, and detection platform
• Detection: Pair with streptavidin-HRP, streptavidin-AP, or streptavidin-fluorophores
• Fixation: Compatible with aldehyde-based fixatives for IHC (optimize epitope preservation as needed)

What You Get

• 50 g alpha-Endorphin Antibody conjugated to Biotin in solution
• High-affinity biotinylated reagent for sensitive streptavidin-based detection
• Biotechnology-grade antibody suitable for opioid peptide and neuroendocrine research
• Batch-specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Specific recognition of -endorphin (alpha-endorphin)
• Biotin conjugation enables flexible, high-sensitivity streptavidin amplification
• Excellent for mapping endorphin distribution and quantifying opioid peptides
• Supports studies in pain modulation, stress response, and neuroendocrine signaling
• High-purity biotechnology-grade formulation

Frequently Asked Questions (FAQ)

• What does this antibody specifically detect?
  It is directed against -endorphin (alpha-endorphin), an endogenous opioid peptide derived from POMC.
• What detection systems are compatible?
  The biotin tag allows pairing with any streptavidin-conjugated reporter (HRP, AP, fluorophores, or magnetic beads).
• What is the recommended storage condition?
  Store at -20 C. Prepare aliquots to avoid repeated freezethaw cycles.
• Is this antibody suitable for immunohistochemistry?
  Yes. It is commonly used for IHC to visualize -endorphin in brain sections and neuroendocrine tissues.
• Can it be used for quantification of -endorphin?
  Yes, when combined with streptavidin-HRP in ELISA or radioimmunoassay formats.

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

References

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• Nagahama M, Funasaka Y, Fernandez-Frez ML, Ohashi A, Chakraborty AK, Ueda M, Ichihashi M. Immunoreactivity of alpha-melanocyte-stimulating hormone, adrenocorticotrophic hormone and beta-endorphin in cutaneous malignant melanoma and benign melanocytic naevi Br J Dermatol. 1998 Jun;138(6):981-5.
• Wu HY, Tang XQ, Mao XF, Wang YX. Autocrine Interleukin-10 Mediates Glucagon-Like Peptide-1 Receptor-Induced Spinal Microglial -Endorphin Expression J Neurosci. 2017 Nov 29;37(48):11701-11714.
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