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Native Human Proteinase 3

Native Human Proteinase 3

Original price was: $145.00.Current price is: $36.25.

SKU: PW14836849480 Category:

Description

Native Human Proteinase 3 Catalog number: B2017446 (2 g)

Catalog number: B2017446
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2 g
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: 2-8C
Keywords: ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP-4, NP4, P29, PR-3, PR3
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um.

Native Human Proteinase 3 (PR3) is supplied as a solution in a defined 2g amount (Catalog B2017446) with storage specified at 28C. PR3 is a serine protease prominently associated with the neutrophil lineage and has long served as a research reagent in studies that examine protease behavior, processing of peptide substrates, and immune-system related mechanisms. In keeping with the suppliers listing, this product is positioned as a molecular tool for various biochemical applications. Paired with explicit documentation fields (amount, supplied-as, storage, and keyword synonyms), the format supports straightforward inclusion in SOPs and inventory systems where traceability and reproducible handling are required.

When method development calls for a native serine protease benchmark, PR3 offers a well-characterized enzyme context for exploratory protocols such as qualitative detection, binding evaluations, or workflow checks that probe buffer compatibility and temperature sensitivity. Because it is provided ready to dilute, users can conduct pilot titrations directly in working buffers to map signal windows and background behavior without reconstitution steps. The concise specification set encourages recording of lot identifiers, diluent composition, and time-at-temperature during optimization so conditions can be reproduced across runs, analysts, and platforms. The presence of recognized synonyms in the keyword line (e.g., PR-3/PR3, C-ANCA-related terms, historical designations such as NP-4 and P29) helps align internal search, LIMS entries, and cross-references in multi-team environments.

Typical use cases include assembling proof-of-concept capture/detection elements, establishing internal references for qualitative comparisons, and assessing sample-prep variables that influence protease activity or stability. Because the listing does not declare a specific concentration, it is prudent to determine working dilutions empiricallyin small-scale matrices firstbefore scaling to routine experiments. Within these boundaries, Native Human Proteinase 3 functions as a reliable, clearly specified component for research workflows that examine serine protease systems or need a native PR3 preparation for exploratory biochemical investigations.

Why researchers choose this product:

  • Defined 2g amount in a ready-to-dilute solution format
  • Specified storage at 28C supports routine cold-chain handling
  • Positioned as a molecular tool for diverse biochemical applications
  • Keyword synonyms (e.g., PR-3/PR3, C-ANCA terms, NP-4/P29) aid searchability and SOP alignment
  • Grade note specifies Type I water and 0.22m filtration for solutions

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • 1: Surmiak M, Kaczor M, Sanak M. Proinflammatory genes expression in granulocytes activated by native proteinase-binding fragments of anti-proteinase 3 IgG J Physiol Pharmacol. 2015 Aug;66(4):609-15.
  • 2: Damoiseaux J, Dhnrich C, Rosemann A, Probst C, Komorowski L, Stegeman CA, Egerer K, Hiepe F, van Paassen P, Stcker W, Schlumberger W, Tervaert JW. A novel enzyme-linked immunosorbent assay using a mixture of human native and recombinant proteinase-3 significantly improves the diagnostic potential for antineutrophil cytoplasmic antibody-associated vasculitis Ann Rheum Dis. 2009 Feb;68(2):228-33.
  • 3: Khorsand F, Haug BE, Kursula I, Reuter N, Brenk R. Expression and purification of human neutrophil proteinase 3 from insect cells and characterization of ligand binding PLoS One. 2024 Jun 25;19(6):e0294827.
    4: Traum A, Hofmann C, Haas S, Schmidt S, Bein G, Sachs UJ, Bayat B. Characterization of CD177-reactive iso- and auto-antibodies Transfusion. 2021 Jun;61(6):1916-1922.
  • 5: Jenne DE, Kuhl A. Production and applications of recombinant proteinase 3, Wegeners autoantigen: problems and perspectives Clin Nephrol. 2006 Sep;66(3):153-9.
    6: Relle M, Mayet WJ, Strand D, Brenner W, Galle PR, Schwarting A. Proteinase 3/myeloblastin as a growth factor in human kidney cells J Nephrol. 2003 Nov-Dec;16(6):831-40.
  • 7: Tongaonkar P, Golji AE, Tran P, Ouellette AJ, Selsted ME. High fidelity processing and activation of the human -defensin HNP1 precursor by neutrophil elastase and proteinase 3 PLoS One. 2012;7(3):e32469.
    8: Zoega M, Trier NH, Nejrup RG, Chailyan A, Friis T, Hjrup P, Houen G. Defensin Interactions in Relation to Monoclonal and Disease-Related Proteinase 3 Antibodies Binding at the Catalytic Site Antibodies (Basel). 2023 Mar 13;12(1):23.
  • 9: Zhou Z, Richard C, Mnard HA. De novo synthesis of proteinase 3 by cytokine primed circulating human polymorphonuclear neutrophils and mononuclear cells J Rheumatol. 2000 Oct;27(10):2406-11.
    10: Rao NV, Marshall BC, Gray BH, Hoidal JR. Interaction of secretory leukocyte protease inhibitor with proteinase-3 Am J Respir Cell Mol Biol. 1993 Jun;8(6):612-6. pubmed.ncbi.nlm.nih.gov

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