Description
alpha-Endorphin Antibody Conjugated to Biotin Research Use Only
alpha-Endorphin Antibody Conjugated to Biotin is a biotinylated polyclonal antibody specific for -endorphin, an endogenous opioid peptide derived from pro-opiomelanocortin (POMC). Supplied as a 50 g solution, this high-affinity biotin conjugate enables sensitive streptavidin-based detection of -endorphin in immunoassays, immunohistochemistry, and neuroendocrine research.
| Catalog number: | B2015909 |
| Lot number: | Batch dependent |
| Expiration Date: | Batch dependent |
| Amount: | 50 g |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Solution |
| Applications: | ELISA, radioimmunoassay (RIA), immunohistochemistry (IHC), immunocytochemistry, and streptavidin-biotin detection of -endorphin |
| Storage: | -20 C |
| Keywords: | alpha-Endorphin, Endorphin, Endorphin a |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
Scientific Overview
-Endorphin is an endogenous opioid peptide generated from the precursor pro-opiomelanocortin (POMC). It exhibits opioid activity primarily through – and -opioid receptors and is involved in pain modulation, stress response, and neuroendocrine regulation. The biotinylated antibody allows high-sensitivity detection and localization of -endorphin in neural tissues, plasma, and other biological samples using streptavidin-based amplification systems.
This alpha-Endorphin Antibody Biotin Conjugate is suitable for:
- Immunohistochemical mapping of -endorphin in the central nervous system
- Quantification of -endorphin by ELISA or radioimmunoassay
- Studies of opioid peptide systems, stress axis, and pain pathways
- Investigation of endorphin immunoreactivity in physiological and pathological conditions
- Neuropeptide co-localization and neuroendocrine research
Usage & Handling Guidance
Store the antibody solution at -20 C. Avoid repeated freezethaw cycles by preparing single-use working aliquots. Thaw gently on ice and mix by gentle inversion before use. Dilute in buffer containing carrier protein (e.g., 1% BSA) immediately prior to assay.
- Recommended applications: ELISA, RIA, IHC, and streptavidin-biotin detection systems
- Working dilution: Optimize empirically for each tissue, fixation method, and detection platform
- Detection: Pair with streptavidin-HRP, streptavidin-AP, or streptavidin-fluorophores
- Fixation: Compatible with aldehyde-based fixatives for IHC (optimize epitope preservation as needed)
What You Get
- 50 g alpha-Endorphin Antibody conjugated to Biotin in solution
- High-affinity biotinylated reagent for sensitive streptavidin-based detection
- Biotechnology-grade antibody suitable for opioid peptide and neuroendocrine research
- Batch-specific documentation available upon request
- For research use only (RUO)
Why Researchers Choose It
- Specific recognition of -endorphin (alpha-endorphin)
- Biotin conjugation enables flexible, high-sensitivity streptavidin amplification
- Excellent for mapping endorphin distribution and quantifying opioid peptides
- Supports studies in pain modulation, stress response, and neuroendocrine signaling
- High-purity biotechnology-grade formulation
Frequently Asked Questions (FAQ)
- What does this antibody specifically detect?
It is directed against -endorphin (alpha-endorphin), an endogenous opioid peptide derived from POMC. - What detection systems are compatible?
The biotin tag allows pairing with any streptavidin-conjugated reporter (HRP, AP, fluorophores, or magnetic beads). - What is the recommended storage condition?
Store at -20 C. Prepare aliquots to avoid repeated freezethaw cycles. - Is this antibody suitable for immunohistochemistry?
Yes. It is commonly used for IHC to visualize -endorphin in brain sections and neuroendocrine tissues. - Can it be used for quantification of -endorphin?
Yes, when combined with streptavidin-HRP in ELISA or radioimmunoassay formats.
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.
References
- Bialka S, Sliwczynska M, Marciniak A, Czyzewski D, Misiolek H. An assessment of the effectiveness of regional analgesia after VATS measured by an objective method for assessing testosterone, cortisol, -amylase, sIgA, and -endorphin levels a randomised controlled trial Endokrynol Pol. 2021;72(2):133-142.
- Nagahama M, Funasaka Y, Fernandez-Frez ML, Ohashi A, Chakraborty AK, Ueda M, Ichihashi M. Immunoreactivity of alpha-melanocyte-stimulating hormone, adrenocorticotrophic hormone and beta-endorphin in cutaneous malignant melanoma and benign melanocytic naevi Br J Dermatol. 1998 Jun;138(6):981-5.
- Wu HY, Tang XQ, Mao XF, Wang YX. Autocrine Interleukin-10 Mediates Glucagon-Like Peptide-1 Receptor-Induced Spinal Microglial -Endorphin Expression J Neurosci. 2017 Nov 29;37(48):11701-11714.
- Weber E, Barchas JD. Immunohistochemical distribution of dynorphin B in rat brain: relation to dynorphin A and alpha-neo-endorphin systems Proc Natl Acad Sci U S A. 1983 Feb;80(4):1125-9.
- Gramsch C, Meo T, Riethmller G, Herz A. Binding characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides J Neurochem. 1983 May;40(5):1220-6.
- Winther Bach F, Ekman R, Jensen FM. Beta-endorphin-immunoreactive components in human cerebrospinal fluid Regul Pept. 1986 Dec 30;16(3-4):189-98.
- Johnson HM, Smith EM, Torres BA, Blalock JE. Regulation of the in vitro antibody response by neuroendocrine hormones Proc Natl Acad Sci U S A. 1982 Jul;79(13):4171-4.
- Berczi I, Chalmers IM, Nagy E, Warrington RJ. The immune effects of neuropeptides Baillieres Clin Rheumatol. 1996 May;10(2):227-57.
- Liu H, He Y, Beck J, da Silva Teixeira S, Harrison K, Xu Y, Sisley S. Defining vitamin D receptor expression in the brain using a novel VDR(Cre) mouse J Comp Neurol. 2021 Jun;529(9):2362-2375.
