Description
Protein A Turbo Proximity Biotinylation Enzyme (RUO)
Protein A Turbo is a biotechnologygrade proximity biotinylation enzyme designed for rapid and efficient labeling of proteins in spatial proximity to antibodybound targets. By fusing Protein A to TurboID, this reagent enables offtheshelf proximity labeling without the need for genetic engineering of the target protein. When combined with a primary antibody, Protein A Turbo recruits TurboID to the protein of interest, allowing biotinylation of nearby interactors for downstream enrichment and proteomic analysis. This approach supports interaction proteomics, chromatinassociated protein mapping, and antibodyguided proximity labeling workflows.
| Catalog number: | B2018844 |
| Lot number: | Batch dependent |
| Expiration Date: | Batch dependent |
| Amount: | 50 g |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Solution |
| Applications: | Proximity labeling, interaction proteomics, antibodyguided TurboID workflows |
| Storage: | -80 C |
| Keywords: | Protein A Turbo, ProtATurboID, proximity biotinylation enzyme, TurboID labeling |
| Grade: | Biotechnology grade. Prepared with Type I ultrapure water (>18 Mcm) and sterilefiltered (0.22 m). |
Scientific Overview
Protein A Turbo enables antibodydirected proximity biotinylation by coupling the highaffinity IgGbinding properties of Protein A with the rapid labeling kinetics of TurboID. Upon addition of biotin, TurboID catalyzes the formation of reactive biotinAMP, which covalently labels lysine residues on nearby proteins. This allows researchers to map proteinprotein interactions, chromatinassociated complexes, and spatially localized proteomes without genetic fusion of TurboID to the target protein. The method is compatible with fixed or permeabilized cells, isolated nuclei, and biochemical preparations.
Common Research Applications
- Antibodyguided proximity labeling using TurboID
- Interaction proteomics and protein complex mapping
- Chromatinassociated protein identification
- Spatial proteomics and subcellular protein profiling
- Antibodybased enrichment of protein microenvironments
- Proximity labeling in cells, nuclei, or biochemical extracts
Usage & Handling Guidance
Store at -80 C. Thaw on ice and aliquot immediately to avoid freezethaw cycles. Use lowbinding tubes and maintain cold conditions during handling. For optimal performance, combine Protein A Turbo with a validated primary antibody and add biotin to initiate labeling.
- Storage: -80 C
- Handling: Aliquot upon receipt; avoid freezethaw cycles
- Compatibility: TurboID workflows, proteomics, proximity labeling assays
What You Get
- 50 g Protein A Turbo (Proximity Biotinylation Enzyme)
- Highpurity biotechnologygrade preparation
- Batchspecific documentation available upon request
- For research use only (RUO)
Frequently Asked Questions
- How does Protein A Turbo work?
Protein A binds to the Fc region of IgG antibodies, positioning TurboID near the antibodybound target. Upon biotin addition, TurboID biotinylates nearby proteins for downstream enrichment. - Do I need to genetically modify my target protein?
No. Protein A Turbo enables proximity labeling using only a primary antibody, eliminating the need for genetic fusion constructs. - What types of samples are compatible?
Protein A Turbo can be used in cells, permeabilized cells, isolated nuclei, and biochemical extracts, depending on the workflow. - Can this reagent be used for chromatinassociated protein mapping?
Yes. Antibodyguided TurboID labeling is widely used for chromatinassociated proteomics. - How should the enzyme be stored?
Store at -80 C and aliquot to avoid freezethaw cycles. - Is this reagent suitable for diagnostic or therapeutic use?
No. This product is intended strictly for in vitro research use only (RUO). - Can I request a COA or TDS?
Yes. COA/TDS availability is batch dependent. Contact us with the catalog and lot number.
References
- SantosBarriopedro I, van Mierlo G, Vermeulen M. Offtheshelf proximity biotinylation using ProtATurboID. Nat Protoc. 2023.
- SantosBarriopedro I, van Mierlo G, Vermeulen M. Offtheshelf proximity biotinylation for interaction proteomics. Nat Commun. 2021.
- Cho KF et al. Proximity labeling with TurboID and splitTurboID. Nat Protoc. 2020.
- Cho KF et al. SplitTurboID enables contactdependent labeling. PNAS. 2020.
- May DG et al. Comparative application of BioID and TurboID. Cells. 2020.
- Kido K et al. AirID proximity labeling enzyme. eLife. 2020.
