Description
Recombinant F. heparinum Heparinase I Protein
| Catalog number: | B2012866 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 10 ug |
| Molecular Weight or Concentration: | 42 kDa |
| Supplied as: | Solution |
| Applications: | molecular tool for various biochemical applications |
| Storage: | -20C |
| Keywords: | Recombinant F. heparinum Heparinase I Protein, CF |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Ernst S, Venkataraman G, Winkler S, Godavarti R, Langer R, Cooney CL, Sasisekharan R. Expression in Escherichia coli, purification and characterization of heparinase I from Flavobacterium heparinum Biochem J. 1996 Apr 15;315 ( Pt 2)(Pt 2):589-97.
- Huang J, Cao L, Guo W, Yuan R, Jia Z, Huang K. Enhanced soluble expression of recombinant Flavobacterium heparinum heparinase I in Escherichia coli by fusing it with various soluble partners Protein Expr Purif. 2012 Jun;83(2):169-76.
- Galliher PM, Cooney CL, Langer R, Linhardt RJ. Heparinase production by Flavobacterium heparinum Appl Environ Microbiol. 1981 Feb;41(2):360-5.
- Ma X, Wang Z, Li S, Shen Q, Yuan Q. Effect of CaCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Nov 7;843(2):209-15.
- Warnement CM, Cismowski MJ, Rogers LK. Optimizing miR-29 measurements in biobanked, heparinized samples Life Sci. 2019 Dec 1;238:116894.
- Watanabe M, Tsuda H, Yamada S, Shibata Y, Nakamura T, Sugahara K. Characterization of heparinase from an oral bacterium Prevotella heparinolytica J Biochem. 1998 Feb;123(2):283-8.
- Luo Y, Huang X, McKeehan WL. High yield, purity and activity of soluble recombinant Bacteroides thetaiotaomicron GST-heparinase I from Escherichia coli Arch Biochem Biophys. 2007 Apr 1;460(1):17-24.
- Blain F, Tkalec AL, Shao Z, Poulin C, Pedneault M, Gu K, Eggimann B, Zimmermann J, Su H. Expression system for high levels of GAG lyase gene expression and study of the hepA upstream region in Flavobacterium heparinum J Bacteriol. 2002 Jun;184(12):3242-52.
- Godavarti R, Sasisekharan R. A comparative analysis of the primary sequences and characteristics of heparinases I, II, and III from Flavobacterium heparinum Biochem Biophys Res Commun. 1996 Dec 24;229(3):770-7.
