Description
Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.
| Catalog number: | B2019702 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 250 M IU |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Solution |
| Applications: | a molecular tool for various biochemical applications |
| Storage: | 20C |
| Keywords: | SCDase |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Han YB, Wu L, Rich JR, Huang FT, Withers SG, Feng Y, Yang GY. Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids Appl Microbiol Biotechnol. 2015 Aug;99(16):6715-26.
- Kuchar L, Rotkov J, Asfaw B, Lenfeld J, Hork D, Koreck L, Blkov Z, Ledvinov J. Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry Rapid Commun Mass Spectrom. 2010 Aug 30;24(16):2393-9.
- Ito M, Kurita T, Kita K. A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms J Biol Chem. 1995 Oct 13;270(41):24370-4.
- Okino N, Ito M. Hydrolysis of glycosphingolipids by a sphingolipid ceramide N-deacylase (SCDase) 2021 Sep 28 [updated 2022 Mar 18]. In: Nishihara S, Angata K, Aoki-Kinoshita KF, Hirabayashi J, editors. Glycoscience Protocols (GlycoPODv2) [Internet]. Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021.
- Tirodkar TS, Voelkel-Johnson C. Sphingolipids in apoptosis Exp Oncol. 2012 Oct;34(3):231-42.
- Mitsutake S, Kita K, Okino N, Ito M. [14C]ceramide synthesis by sphingolipid ceramide N-deacylase: new assay for ceramidase activity detection Anal Biochem. 1997 Apr 5;247(1):52-7.
- Cho KW. Bioluminescent assay for sphingolipid ceramide N-deacylase using Vibrio harveyi dark mutant M-17 J Microbiol. 2008 Oct;46(5):585-9.
- Furusato M, Sueyoshi N, Mitsutake S, Sakaguchi K, Kita K, Okino N, Ichinose S, Omori A, Ito M. Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8 J Biol Chem. 2002 May 10;277(19):17300-7.
- Nakagawa T, Tani M, Kita K, Ito M. Preparation of fluorescence-labeled GM1 and sphingomyelin by the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase as substrates for assay of sphingolipid-degrading enzymes and for detection of sphingolipid-binding proteins J Biochem. 1999 Sep;126(3):604-11.
